The purification and properties of rabbit-muscle myokinase.
نویسنده
چکیده
Bryant, M. P. & Small, N. (1956). J. Bad. 72, 16. Doetsch, R. N., Howard, B. H., Mann, S. 0. & Oxford, A. E. (1957). J. gen. Microbiol. 16, 156. Drury, H. F. (1948). Arch. Biochem. 19, 455. Duncan, W. A. M., Manners, D. J. & Ross, A. G. (1954). Biochem. J. 57, xviii. Duncan, W. A. M., Manners, D. J. & Ross, A. G. (1956). Biochem. J. 63, 44. Elsden, S. R. (1946). J. exp. Biol. 22, 51. Finan, P. A. & O'Colla, P. S. (1955). CJhem. &Ind. p. 1387. Heald, P. J. (1952). Biochem. J. 50, 503. Heald, P. J. (1953). Brit. J. Nutr. 7, 124. Hirst, E. L. (1955). J. chem. Soc. p. 2974. Hirst, E. L., Hough, L. & Jones, J. K. N. (1949). J. chem. Soc. p. 928. Howard, B. H. (1955). Biochem. J. 60, i. Kunkel, H. G. & Tiselius, A. (1951). J. gen. Phy8iol. 85, 89. Marshall, R. A. (1949). Brit. J. Nutr. 3, 1. Mould, D. L. & Synge, R. L. M. (1954). Biochem. J. 58,585. Percival, E. G. V. & Chanda, S. K. (1950). Nature, Lond., 168, 787. Perlin, A. S. (1951 a). Cereal chem. 28, 370. Perlini, A. S. (1951 b). Cereal chem. 28, 382. Perlin, A. S. (1954). J. Amer. chem. Soc. 76,4101. Somogyi, M. (1945a). J. biol. Chem. 160, 61. Somogyi, M. (1945b). J. biol. Chem. 160, 69. Whistler, R. L. & Durso, D. F. (1950). J. Amer. chem. Soc. 72, 677. Whistler, R. L. & Tu, C. C. (1951). J. Amer. chem. Soc. 73, 1389. Whistler, R. L. & Tu, C. C. (1952). J. Amer. chem. Soc. 74, 3609. Whistler, R. L. & Tu, C. C. (1953). J. Amer. chem. Soc. 75, 645.
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The mechanism of myokinase (ATP-AMP phosphotransferase, EC 2.7.4.3) from rabbit muscle has been investigated by studies of equilibrium isotope exchange and initial velocities. The mechanism for the myokinase reaction is random bi bi. Under most conditions, equilibrium isotope exchange between AMP and ADP is faster than that between ATP and ADP. The rate-limiting steps in the reaction include th...
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 67 4 شماره
صفحات -
تاریخ انتشار 1957